Main Article Content

Fathin Hamida
Vilya Syafriana
Carla Febriayu Ramadhani
Elsa Vera Nanda

Abstract

ABSTRAK


Streptococcus mutans merupakan salah satu bakteri penyebab karies gigi. Penggunaan antibiotic merupakan terapi infeksi yang umum dilakukan untuk menghambat pertumbuhan bakteri. Biji anggur diketahui mengandung senyawa polifenol yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas ektrak biji anggur terhadap Streptococcus mutans. Bahan uji yang digunakan adalah buah anggur yang diambil bijinya. Buah anggur diperoleh dari Pasar Induk, Kramat Jati, Jakarta Timur. Ekstrak biji anggur diperoleh dengan metode maserasi menggunakan pelarut etil asetat dan etanol 70%. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram pada media Mueller Hinton Agar dengan konsentrasi 5%, 10%, 20%, dan 40%. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% biji anggur memiliki aktivitas antibakteri pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 8,46 mm; 8,91 mm; 9,89 mm; dan 11,04 mm. Hasil pada ekstrak etil asetat juga menunjukkan ada aktivitas penghambatan pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 7,72 mm; 8,50 mm; 9,64 mm; dan 10,51 mm. Ekstrak etanol 70% biji anggur memiliki daya hambat yang lebih besar dibandingkan dengan ekstrak etil asetat. Hasil ini dapat menjadi acuan untuk pengembangan potensi biji anggur sebagai bahan obat atau kosmedik (kosmetik-medik) penanganan karies gigi.


 


ABSTRACT


Streptococcus mutans is one of the bacteria that cause dental caries. The antibiotics usually used for the therapy of this infection to inhibit bacterial growth. Polyphenol, which recommended as an antibacterial agent, is common secondary metabolites in grape seeds. The purpose of this study is to know the activity of grape seed extract against Streptococcus mutans. The grape seeds were obtained from fresh fruits which bought from Pasar Induk, Kramat Jati, East Jakarta. The grape seed extract was obtained by the maceration method using ethyl acetate and 70% ethanol. The antibacterial activity test was carried out using the disk diffusion method on Mueller Hinton Agar media with a concentration of 5%, 10%, 20%, and 40%. The results showed that 70% ethanol extract of grape seeds had antibacterial activity at concentrations of 5%, 10%, 20%, and 40% with a value of 8.46 mm; 8.91 mm; 9.89 mm; and 11.04 mm respectively. The results of ethyl acetate extract also showed inhibitory activity at concentrations of 5%, 10%, 20%, and 40% with values ​​of 7.72 mm; 8.50 mm; 9.64 mm; and 10.51 mm respectively. The inhibition of 70% ethanol extract of grape seed is greater than ethyl acetate extract. The results of the study can be used as reference for the potential development of grape seed as a medicinal or cosmedic cosmetic-medical) ingredient for treating dental caries.

Article Details

References
[1] Kementerian Kesehatan Badan Penelitian dan Pengembangan Kesehatan. RISKESDAS 2013: Riset Kesehatan Dasar 2013. [Diakses 3 Mei 2021]. Tersedia dari: https://pusdatin.kemkes.go.id/resources/download/general/Hasil%20Riskesdas%202013.pdf

[2] Kementerian Kesehatan Badan Penelitian dan Pengembangan Kesehatan. Hasil Utama RISKESDAS 2018. [Diakses 3 Mei 2021]. Tersedia dari: https://www.litbang.kemkes.go.id/hasil-utama-riskesdas-2018/

[3] Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients. 2010;2(3):290–298. https://doi.org/10.3390/nu2030290

[4] Zhao W, Xie Q, Bedran-Russo AK, Pan S, Ling J, Wu CD. The preventive effect of grape seed extract on artificial enamel caries progression in a microbial biofilm-induced caries model. Journal of Dentistry. 2014;42(8):1010–1018. https://doi.org/10.1016/j.jdent.2014.05.006

[5] Khodadadi E, Moghadamnia AA, Rajabnia R, Khafri S, Zarei R. The effect of different Vitis vinifera seed extracts on Streptococcus mutans and sobrinus bacteria. Journal of Dentomaxillofacial Radiology, Pathology and Surgery. 2019;8(1):1-15.

[6] Paul RK, Dutta D, Chakraborty D, Nayak A, Dutta PK, Nag M. Antimicrobial agents from natural sources: An overview. Advance Pharmaceutical Journal. 2019;4(2):41-51.

[7] Sadeek AM, Abdallah EM. Phytochemical compounds as antibacterial agents: A mini review. Saudi Arabia Glob J Pharmaceu Sci. 2019;7(4):001-006. doi: 10.19080/GJPPS.2019.07.555720.

[8] Georgiev V, Ananga A, Tsolova V. Recent advances and uses of grape flavonoids as nutraceuticals. Nutrients. 2014;6:391-415.

[9] Krithika V, Naik R, Pragalyaashree. Functional properties of grape (Vitis vinifera) seed extract and possible extraction techniques - A review. Agri. Review. 2015;36 (4):313-320.

[10] Fadillah CY, Al-Mukholladun AW, Syafriana V. Aktivitas antifungi ekstrak etanol biji anggur (Vitis vinifera L.) terhadap Candida albicans. Sainstech Farma. 2017;10(1):25-29.

[11] Syafriana V, Hamida F, Puspita D, Haryani F, Nanda EV. Aktivitas antifungi ekstrak etanol biji anggur terhadap Malassezia furfur dan Trichophyton mentagrophytes. Bioma. 2020a;16(1):21-30.

[12] Syafriana V, Hamida F, Damayanti R, Nanda EV. Aktivitas antibakteri ekstrak biji anggur (Vitis vinifera L.) terhadap Streptococcus pyogenes. Sainstech Farma. 2020b;13(1):40-44.

[13] Syafriana V, Hamida F, Nanda EV, Laili N, Aslamiyah. Aktivitas antibakteri ekstrak n-heksana dan etanol biji anggur terhadap Staphylococcus epidermidis dan Propionibacterium acnes. Prosiding Seminar Nasional Biologi di Era Pandemi COVID-19; 19 September; Gowa, Indonesia. Indonesia: UIN Alaudin Makassar. 2020c.

[14] Departemen Kesehatan Republik Indonesia. Materia Medika Indonesia Jilid VI. Direktorat Pengawasan Obat dan Makanan. Jakarta. 1995. p. 333-337.

[15] Pandey A, Tripathi S. Concept of standardization, extraction and pre-phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry. 2014;2(5):115-119.

[16] Hogg S. Essential Microbiology. West Sussex: John Wiley & Sons Ltd. 2005. p. 367—368.

[17] Hudzicki J. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. American Society for Microbiology. 2016. [diakses pada 26 Agustus 2018]. Available at: https://asm.org/getattachment/2594ce26-bd44-47f6-8287-0657aa9185ad/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test-Protocol-pdf.pdf

[18] Azwanida NN. A review on the extraction methods use in medicinal plants. Principle, Medicinal & Aromatic Plants. 2015;4(3):1-6.

[19] Zhang Q-W, Lin L-G, Ye W-C. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13(20):1-26.

[20] Velavan S. Phytochemical Techniques - A Review. World Journal of Science and Research. 2015;1(2):80-91

[21] Joshi DR, Adhikari N. An Overview on common organic solvents and their toxicity. Journal of Pharmaceutical Research International. 2019;June:1–18. doi: 10.9734/jpri/2019/v28i330203.

[22] Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009;14(6):2167–2180. https://doi.org/10.3390/molecules14062167.

[23] Noviyanty A, Salingkat CA. Pengaruh jenis pelarut terhadap ekstraksi dari kulit buah naga merah (Hylocereus polyrhizus). Kovalen. 2019;5(3):271–279.

[24] Felhi S, Daoud A, Hajlaoui H, Mnafgui K, Gharsallah N, Kadri A. Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits. Food Science and Technology. 2017;37(3):483–492. https://doi.org/10.1590/1678-457x.23516

[25] Buchari ET, Sulaeman A. Pengaruh pelarut dan temperatur terhadap transport europium (III) melalui membran cair berpendukung. Jurnal Matematika dan Sains. 2003;8(4):155.

[26] Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia. 2011;1(1):98-106.

[27] Sari PP, Rita WS, Puspawati NM. Identifikasi dan uji aktivitas senyawa tanin dari ekstrak daun trembesi (Samanea saman (Jacq.) Merr) sebagai antibakteri Escherichia coli (E. coli). Jurnal Kimia (Journal of Chemistry). 2015;9(1):27-34.

[28] Waghorn GC, McNabb WC. Consequences of plant phenolic compounds for productivity and health of ruminants. Proc. Nutr. Soc. 2003;62:383-392.

[29] Aguilera-Carbo A, Augur C, Prado-Barragan LA, Favela-Torres E, Aguilar CN. Microbial production of ellagic acid and biodegradation of ellagitannins. Applied Microbiology and Biotechnology. 2008;78(2):189–199. https://doi.org/10.1007/s00253-007-1276-2

[30] Ratnaningtyas NI, Purnomowati P, Purwati ES, Septiana AT, Ekowati N, Supriyadi A. Antioxidant potential of ethanol and ethyl acetate extract of Ganoderma sp. mycelium. Biosaintifika: Journal of Biology & Biology Education. 2018;10(1):87-94.

[31] Dewi S, NYRS Asseggaf S, Natalia D. Efek ekstrak etanol daun kesum (Polygonum minus Huds.) sebagai antifungi terhadap Trichophyton rubrum. Jurnal Kesehatan Andalas. 2019;8(2):198–203. Retrieved from http://jurnal.fk.unand.ac.id

[32] Lingga AR, Pato U, Rossi E. Uji antibakteri ekstrak batang kecombrang (Nicolaia speciosa Horan) terhadap Staphylococcus aureus dan Escherichia coli. JOM Faperta. 2016;3(1):1-15.

[33] Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12(4):564 – 582.

[34] Fitriah, Mappiratu, Prismawiranti. Uji aktivitas antibakteri ekstrak daun tanaman johar (Cassia siamea Lamk.) dari beberapa tingkat kepolaran pelarut. Jurnal Riset Kimia. 2017;3(3):249.

[35] Ibrahim A, Kuncoro. Identifikasi metabolit sekunder dan aktivitas antibakteri ekstrak daun sungkai (Peronema canescens Jack) terhadap beberapa bakteri patogen. Journal of Tropical Pharmacy and Chemistry. 2012;2(1):8-18.

[36] Górniak I, Bartoszewski R, Króliczewski J. Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews. 2019;18(1):241–272. https://doi.org/10.1007/s11101-018-9591-z

[37] Khameneh B, Iranshahy M, Soheili V, Bazzaz BSF. Review on plant antimicrobials: A mechanistic viewpoint. Antimicrobial Resistance and Infection Control. 2019;8:1-28.