POTENSI ANTIOKSIDAN CLAY MASK STICK BERBASIS RICE BRAN OIL TERHADAP RADIKAL BEBAS DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)
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Mar 25, 2026
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Abstract
Masalah kesehatan kulit wajah menjadi perhatian penting, terutama bagi perempuan, karena berpengaruh terhadap rasa percaya diri dan penampilan. Salah satu upaya perawatan kulit wajah adalah penggunaan masker berbahan alami yang dinilai lebih aman dan ramah lingkungan. Seiring berkembangnya inovasi di bidang kosmetik, sediaan clay mask stick hadir sebagai bentuk baru yang lebih praktis digunakan. Penelitian ini bertujuan untuk mengevaluasi potensi antioksidan dari clay mask stick yang diformulasikan dengan minyak bekatul (rice bran oil/RBO) menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Minyak bekatul diketahui mengandung senyawa antioksidan alami seperti tokoferol, tokotrienol, dan oryzanol. Uji aktivitas antioksidan dilakukan dengan mengukur nilai IC50 menggunakan spektrofotometer UV-Vis pada panjang gelombang 520 nm. Hasil penelitian menunjukkan bahwa formula yang mengandung RBO (F2 dan F3) memiliki nilai IC50 < 50 ppm, yang menunjukkan aktivitas antioksidan yang sangat kuat. Uji ANOVA menunjukkan adanya perbedaan signifikan antar formula (p = 0,003), dan uji lanjut LSD membuktikan bahwa formula F2, F3, serta pembanding vitamin C secara signifikan berbeda dari formula kontrol (F0). Selain itu, hasil uji karakteristik fisik (pH, homogenitas, daya sebar, dan waktu kering) menunjukkan bahwa seluruh sediaan memenuhi standar mutu kosmetik yang baik. Kesimpulannya, clay mask stick berbasis rice bran oil memiliki potensi kuat sebagai produk perawatan kulit topikal dengan aktivitas antioksidan yang efektif dan stabil
Kata kunci: clay mask stick, minyak bekatul, antioksidan, metode DPPH, IC50, tokoferol
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References
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Zhang, Y., et al. (2021). Polyphenols from Grape Pomace: Functionalization of Chitosan Coatings for Enhanced Antioxidant Properties. Langmuir, 37(12), 3535-3544.
Zhao, Y., et al. (2022). Surface functionalization of bioactive glasses and hydroxyapatite with polyphenols: Characterization and antioxidant activity evaluation. Journal of the American Ceramic Society, 105(5), 24582468.
Aithal, K. S., Udupa, N., & Khedkar, S. (2020). Evaluation of spreadability and extrudability of topical formulations. Journal of Applied Pharmaceutical Science, 10(2), 85–89.
Akbarbaglu, Z., Tamjidi, F., & Sarabandi, K. (2024). Production, characterization and spray-drying encapsulation of date (Phoenix dactylifera L.) seed protein hydrolysates for bread fortification. Journal of Food Engineering, 324, 112-120.
Allen, L. V., Popovich, N. G., & Ansel, H. C. (2011). Ansel’s Pharmaceutical Dosage Forms and Drug Delivery Systems (9th ed.). Lippincott Williams & Wilkins.
CNN Indonesia. (2020). 7 sumber antioksidan alami terbaik. CNN Indonesia.
Febriani, Y. Sudewi, S., & Sembiring, R. 2022. Formulation And Antioxcidant Activity Test Of Clay Mask Extracted Ethanol Tamarillo (Solanum Betaceum Cav.). Indonesian Journal Of Pharmaceutical Science And Technology, 1(1), 22. Https://Doi.Org/10.24198/Ijpst.V1i1.36432
Ferraris, S., et al. (2022). Bioactive glasses functionalized with polyphenols: In vitro interactions with healthy and cancerous osteoblast cells. Journal of Materials Science, 57(21), 10056-10067.
Frontiers in Food Science and Technology. (2022). Antioxidant activity of crude rice bran oil by DPPH assay.
Gobbo,V. A. (2020). Biomaterials functionalization with polyphenols and characterization. Politecnico di Torino.
Haki, H. F. (2021). Uji aktivitas antioksidan ekstrak bekatul terfermentasi oleh Rhizopus oryzae (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim).
Harianto, O. A. (2023). Uji toksisitas ekstrak n-heksana bekatul beras merah terhadap larva udang (Artemia salina L.) dengan variasi lama ekstraksi (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim).
Habib, M., Qureshi, I., Nazir, S., Bashir, K., & Jan, K. (2024). Multigrain flour fortification: Enhancing nutritional quality and antioxidant activity in snack products. Food Chemistry, 395, 133-140.
Ju, J., de Oliveira, M.S., & Qiao, Y. (2023). Cinnamon: A medicinal plant and a functional food systems. In Functional Foods (pp. 150-160). Springer Nature Switzerland AG.
Karnila, R., Dewita, E., & Yoswaty, D. (2023). Antioxidant activity on protein hydrolysate peptide of mudskipper fish (Periophthalmodon schlosseri) using alcalase enzyme. Food Science and Technology, 43(2), 123-130.
Kaur, M., & Agrawal, S. (2020). Formulation and evaluation of herbal cosmetic products. Asian Journal of Pharmaceutical and Clinical Research, 13(8), 15–20.
Kaur, M., & Saraf, S. (2021). Organoleptic and physicochemical characterization of cosmetic formulations: A consumer-centered approach. Journal of Cosmetic Science, 72(5), 301–310.
Kittiphattanabawon, P. (2023). Production and cryoprotective effect of hydrolyzed collagen from Nile tilapia prepared by papaya latex enzymes in frozen pacific white shrimp. Thaksin University.
Kumar, A. V. (2022). A novel development of allergen free nutrition bar. Krishikosh.
Kusuma, R., & Amalia, A. (2021). Evaluasi fisik sediaan masker peel-off ekstrak lidah buaya (Aloe vera) terhadap waktu kering dan daya lekat. Jurnal Farmasi Galenika, 7(1), 45–52.
Liu, J. (2024). Valorization of almond hull: Enhancing functional properties and bioavailability as a value-added food ingredient. Journal of Food Science, 89(1), 1-10.
Mahmud, N. (2023). Exploring the effects of citrus peel fiber-enriched batter mix and the delivery of curcumin on the physicochemical properties of oleogel- fried chicken products. 21(4), 345-359.
Majumder, P.K., Kaur, R., Singh, B., & Tripathi, H. (2023). Millets for global human health: A comprehensive analysis of nutritional benefits and health implications of millet consumption worldwide. ResearchGate.
Mingyai, S., Limsuwan, T., Chaiwarit, T., & Chaiwarit, T. (2017). Extraction and antioxidant activity of rice bran oil from different rice cultivars using various extraction methods. Journal of Oleo Science, 66(6), 621–628.
Molyneux, P. (2004). The use of the stable free radical DPPH for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
Mustafa, G., Alotaibi, F. O., & Aldhafiri, F. K. (2021). Ingredients and composition of food grade nanoemulsions. In Nanoemulsions in Food (pp. 45-60). Taylor & Francis Group.
Nicolantonio, L., Ferrati, M., Cristino, M., & Peregrina, D. V. (2023). Evaluation of physicochemical and microbial properties of extracts from wine lees waste of Matelica's Verdicchio and their applications in novel cosmetic products. Antioxidants, 12(5), 456-465.
Puspita, Weni, Dina Yuspita Sari, and Ika Ristia Rahman. "Uji Aktivitas Antioksidan Ekstrak Etanol Daun Buas-buas (Premna serratifolia L.) Asal Kabupaten Melawi Provinsi Kalimantan Barat Dengan Metode DPPH." Jurnal Insan Farmasi Indonesia 3.2 (2020): 405-412.
Rather, M.A., Thakur, R., Hoque, M., & Das, R.S. (2023). Sorghum (Sorghum bicolor): Functional potential and health benefits in human nutrition and health promotion strategies. In Functional Potential (pp. 78-90).
Rowe, R. C., Sheskey, P. J., & Quinn, M. E. (2009). Handbook of Pharmaceutical Excipients (6th ed.). London: Pharmaceutical Press.
Suryadinata, A. (2015). Oil Extraction from Rice Bran with Various Solvents and Concentration of Crude Extract to Antioxidant Activity. ALCHEMY: Journal of Chemistry, 4(1), 25-31.
Saniati, F & Wilujeng, B. . 2020. Analisis Produk Kosmetik Make Up Salah Satu Merek Global Terhadap Keputusan Pembelian. 21(1), 1–9
Shih, Y. T. (2020). Development and characterization of novel muffin products for promoting sustainability. Oregon State University.
Shariatinia, Z. (2020). Biopolymeric nanocomposites in drug delivery: Advances and challenges in the field of drug delivery systems based on biopolymers. In Advanced Biopolymeric Systems for Drug Delivery (pp. 234-245). Springer Nature Switzerland AG.
Sari Mutiara. (2020). 7 Sumber antioksidan alami terbaik. Sari Mutiara.
Setiawan, A., Ulfah, S., & Marlina, N. Formulasi Dan Uji Evaluasi Sediaan Hand & Body Lotion Minyak Bekatul (Rice Bran Oil) Sebagai Antioksidan.
Tarigan, Palas. "FORMULASI SEDIAAN MASKER Gel Peel Off EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L.) SEBAGAI
PEMBERSIH WAJAH." Jurnal Penelitian Farmasi & Herbal 4.2 (2022): 57-62.
Tirado-Kulieva, V. A., et al. (2022). A critical review on the integration of metal nanoparticles in biopolymers: an alternative for active and sustainable food packaging. Research in Food Science, 5(3), 200-
210.
Teschke, R., & Xuan, T. D. (2020). Herbs including shell ginger, antioxidant profiles, aging, and longevity in Okinawa, Japan: A critical analysis of current concepts. Aging, 12(1), 45-56.
Tang, Y., et al. (2023). Functionalization of Ti6Al4V Alloy with Polyphenols:
The Role of the Surface Features. Materials, 13(8), 1347.
Tupperware. (2020). 7 sumberantioksidan alami baik untuk tubuh.Tupperware.
Ulfa, Siti Maria. Identifikasi dan uji aktivitas senyawa antioksidan dalam bekatul dengan menggunakan variasi pelarut. Diss. Universitas Islam Negeri Maulana Malik Ibrahim, 2016.
Wiharsari, J. 2019. Konsep Kecantikan Dan Pemanfaatan Produk Kosmetik Wajah Pada Mahasiswi Surabaya. Jurnal Penelitian Pendidikan Guru Sekolah Dasar, 6(August), 128
Widiyastuti, S., & Nuraini, L. (2022). Evaluasi pH dan efektivitas sediaan masker wajah berbahan dasar alami. Jurnal Ilmu Farmasi dan Sains, 7(1), 34–41.
Yang, L., Wang, H., Yang, Y., & Li, Y. (2024). Self-healing cellulose-based hydrogels: From molecular design to multifarious applications. Carbohydrate Polymers, 240, 116-125.
Yang, Y., Han, J., Qiu, M., He, Y., & Li, C. (2024). Remarks and abstracts of the 14th Asian Congress of Nutrition in Chengdu, China: Highlights and outcomes from the congress discussions on nutrition science advancements and public health implications. Biology and Life Sciences Forum, 14(1), 1- 10.
Younesi, M., Peighambardoust, S. H., & Sarabandi, K. (2023). Application of structurally modified WPC in combination with maltodextrin for microencapsulation of roselle (Hibiscus sabdariffa) extract as a natural colorant source for food products. International Journal of Food Science & Technology, 58(8), 3456-3465.
Yuliansari, M., & Puspitorini, A. 2020. Proses Pembuatan Masker Bunga Rosella Dan Tepung Beras Sebagai Pencerahan Kulit Wajah. Jurnal Tata Rias, 09(Vol 9, No 2 (2020)), 367–376.
Yuliani, S. F., Wahyuni, D., & Susanti, R. (2022). Formulation and evaluation of natural clay mask with rice bran oil and kaolin. Indonesian Journal of Pharmaceutical Science and Technology, 9(2), 123–130.
Zhang, Y., et al. (2021). Polyphenols from Grape Pomace: Functionalization of Chitosan Coatings for Enhanced Antioxidant Properties. Langmuir, 37(12), 3535-3544.
Zhao, Y., et al. (2022). Surface functionalization of bioactive glasses and hydroxyapatite with polyphenols: Characterization and antioxidant activity evaluation. Journal of the American Ceramic Society, 105(5), 24582468.